Impact of glyphosate on the Bradyrhizobium japonicum symbiosis with glyphosate-resistant transgenic soybean: a minireview.
نویسندگان
چکیده
Glyphosate-resistant (GR) soybean [Glycine max (L.) Merr.] expressing an insensitive 5-enolpyruvylshikimic acid-3-phosphate synthase (EPSPS) gene has revolutionized weed control in soybean production. The soybean nitrogen fixing symbiont, Bradyrhizobium japonicum, possesses a glyphosate-sensitive enzyme and upon exposure to glyphosate accumulates shikimic acid and hydroxybenzoic acids such as protocatechuic acid (PCA), accompanied with B. japonicum growth inhibition and death at high concentrations. In a series of greenhouse and field experiments, glyphosate inhibited nodulation and nodule leghemoglobin content of GR soybean. Glyphosate accumulated in nodules of field-grown GR soybean, but its effect on nitrogenase activity of GR soybean was inconsistent in field studies. In greenhouse studies, nitrogenase activity of GR soybean following glyphosate application was transiently inhibited especially in early growth stages, with the greatest inhibition occurring under moisture stress. Studies using bacteroid preparations showed that the level of glyphosate inhibition of bacteroid nitrogenase activity was related to in vitro glyphosate sensitivity of the B. japonicum strains. These studies indicate the potential for reduced nitrogen fixation in the GR soybean system; however, yield reductions due to this reduced N2 fixation in early stages of growth have not been demonstrated.
منابع مشابه
Lack of glyphosate resistance gene transfer from Roundup Ready soybean to Bradyrhizobium japonicum under field and laboratory conditions.
A field study was conducted at the Russell E. Larson Agricultural Research Center to determine the effect of transgenic glyphosate-resistant soybean in combination with herbicide (Roundup) application on its endosymbiont Bradyrhizobium japonicum. DNA of bacteroids from isolated nodules was analysed for the presence of the transgenic 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) DNA se...
متن کاملImpact of glyphosate application to transgenic Roundup Ready soybean on horizontal gene transfer of the EPSPS gene to Bradyrhizobium japonicum and on the root-associated bacterial community
متن کامل
Nitrogenase activity, nitrogen content, and yield responses to glyphosate in glyphosate-resistant soybean
Transgenic glyphosate-resistant (GR) soybean [Glycine max (L.) Merr.] expressing a glyphosate-insensitive 5-enolpyruvylshikimate-3phosphate synthase (EPSPS) enzyme has provided new opportunities for weed control in soybean production. However, glyphosate is toxic to the soybean nitrogen-fixing symbiont, Bradyrhizobium japonicum, as its EPSPS enzyme is sensitive to glyphosate. The effects of gly...
متن کاملThe Probability of a Horizontal Gene Transfer from Roundup Ready® Soybean to Root Symbiotic Bacteria: A Risk Assessment Study on the GSF Lysimeter Station
The gene transfer from glyphosate tolerant soybean to Bradyrhizobium japonicum was evaluated in a free-air lysimeter experiment under natural conditions and increasing selection pressure, to monitor for the probability of horizontal gene transfer (HGT). A large volume lysimeter study that offers conditions comparable to normal farming was conducted in 2004 and 2005 with Roundup Ready® (RR) soyb...
متن کاملA Review on Production of Glyphosate-Resistant Plants with Emphasis on Molecular Methods
Herbicide-resistant plants are one of the most common plants that comprise the majority of the total population of transgenic plants. Glyphosate is an herbicide that controls a wide range of plant species. Position of these herbicides reaction is 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that only exists in plants and some bacteria. This herbicide was known to be suitable and safe for...
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ورودعنوان ژورنال:
- Journal of environmental quality
دوره 33 3 شماره
صفحات -
تاریخ انتشار 2004